Catalog Number
EN010
Product Name
Proteinase K Protein
Alias
Endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine
proteinase, protease K, Tritirachium album proteinase K
Size
1g-1kg, bulk
Species
Engyodontium album
expression Host
Yeast
CAS Number
39450-01-6
Applications
Genomic DNA extraction, Enzyme digestion and removal
Source
This product is derived from yeast cells expressing Proteinase K gene of
Engyodontium album.
Purity
RNase and DNase free.
Endotoxin
None detected
Cross-Reactivity
No cross-reactivity with other antigens or proteins.
Buffer
Dilution Buffer: 20mM Tris-HCl, pH 7.4; Storage buffer: 20mM Tris-HCl, 50%
Glycerol, pH 7.4.
pH Range
4.0-12.0 (Optimum PH 7.5-9.0)
Temperature Range
37-70°C
Specific Activity
≥ 30 units/mg
Molecular Weight
29.3 kDa
Background
Proteinase K is a broad-spectrum serine protease. The enzyme was discovered in
1974 in extracts of the fungus Engyodontium album (formerly Tritirachium
album). Proteinase K is able to digest hair (keratin), hence, the name "Proteinase
K". The predominant site of cleavage is the peptide bond adjacent to the
carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino
groups. It is commonly used for its broad specificity.
Storage
Lyophilized powder: store at 4 °C; Liquid: store at -20 °C.
Shipping Condition
Transport at room temperature
Stability
Lyophilized powder: store at -20 °C for 3 years; Liquid: maintain stable at ambient
temperature for 12 months.
For Research Use Only!